Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes.
Identifieur interne : 001862 ( Main/Exploration ); précédent : 001861; suivant : 001863Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes.
Auteurs : R L Yang [République populaire de Chine] ; A X Wang [République populaire de Chine] ; J. Zhang [République populaire de Chine] ; Y. Dong [République populaire de Chine] ; M S Yang [République populaire de Chine] ; J M Wang [République populaire de Chine]Source :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Bacillus thuringiensis, Populus, Tolérance au sel, Toxines bactériennes.
- méthodes : Lutte biologique contre les nuisibles.
- parasitologie : Populus.
- physiologie : Populus.
- Transgènes.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Bacterial Toxins.
- genetics : Bacillus thuringiensis, Populus, Salt Tolerance.
- methods : Pest Control, Biological.
- parasitology : Populus.
- physiology : Populus.
- Transgenes.
Abstract
We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH) were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x euramericana was transformed by the vector using the Agrobacterium-mediated method. Three transgenic lines were assessed using genetic detection and resistance expression analysis. PCR revealed that exogenous genes Cry1Ac, Cry3A, BADH and selective marker gene NPTII were present in three transgenic lines. Quantitative real-time PCR (qPCR) showed significant differences in the transcriptional abundance of three exogenous genes in different lines. Results of assays for Bt toxic proteins showed that the Cry1Ac and Cry3A toxic protein content of each line was 12.83-26.32 and 2108.91-2724.79 ng/g, respectively. The Cry1Ac toxic protein content of different lines was significantly different; the Cry3A toxic protein content was about 100 times higher than that of the Cry1Ac toxic protein. The insect-resistance test revealed the mortality rate of transgenic lines to Hyphantria cunea L1 larvae varied by 42.2-66.7%, which was significantly higher than non-transgenic lines. The mortality rate of L1 and L2 Plagiodera versicolora larvae was 100%. The insecticidal effect of transgenic lines to P. versicolora larvae was higher than that to H. cunea larvae. NaCl stress tolerance of three transgenic lines under 3-6% NaCl concentration was significantly higher than that of non-transgenic lines.
DOI: 10.4238/gmr.15028635
PubMed: 27173305
Affiliations:
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Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wang, J M" sort="Wang, J M" uniqKey="Wang J" first="J M" last="Wang">J M Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, 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wicri:level="1"><nlm:affiliation>Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wang, A X" sort="Wang, A X" uniqKey="Wang A" first="A X" last="Wang">A X Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, 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Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Y" sort="Dong, Y" uniqKey="Dong Y" first="Y" last="Dong">Y. Dong</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Yang, M S" sort="Yang, M S" uniqKey="Yang M" first="M S" last="Yang">M S Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wang, J M" sort="Wang, J M" uniqKey="Wang J" first="J M" last="Wang">J M Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacillus thuringiensis (genetics)</term><term>Bacterial Toxins (genetics)</term><term>Pest Control, Biological (methods)</term><term>Populus (genetics)</term><term>Populus (parasitology)</term><term>Populus (physiology)</term><term>Salt Tolerance (genetics)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bacillus thuringiensis (génétique)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Populus (génétique)</term><term>Populus (parasitologie)</term><term>Populus (physiologie)</term><term>Tolérance au sel (génétique)</term><term>Toxines bactériennes (génétique)</term><term>Transgènes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Toxins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus thuringiensis</term><term>Populus</term><term>Salt Tolerance</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bacillus thuringiensis</term><term>Populus</term><term>Tolérance au sel</term><term>Toxines bactériennes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Transgènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH) were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x euramericana was transformed by the vector using the Agrobacterium-mediated method. Three transgenic lines were assessed using genetic detection and resistance expression analysis. PCR revealed that exogenous genes Cry1Ac, Cry3A, BADH and selective marker gene NPTII were present in three transgenic lines. Quantitative real-time PCR (qPCR) showed significant differences in the transcriptional abundance of three exogenous genes in different lines. Results of assays for Bt toxic proteins showed that the Cry1Ac and Cry3A toxic protein content of each line was 12.83-26.32 and 2108.91-2724.79 ng/g, respectively. The Cry1Ac toxic protein content of different lines was significantly different; the Cry3A toxic protein content was about 100 times higher than that of the Cry1Ac toxic protein. The insect-resistance test revealed the mortality rate of transgenic lines to Hyphantria cunea L1 larvae varied by 42.2-66.7%, which was significantly higher than non-transgenic lines. The mortality rate of L1 and L2 Plagiodera versicolora larvae was 100%. The insecticidal effect of transgenic lines to P. versicolora larvae was higher than that to H. cunea larvae. NaCl stress tolerance of three transgenic lines under 3-6% NaCl concentration was significantly higher than that of non-transgenic lines. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27173305</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1676-5680</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Apr</Month><Day>29</Day></PubDate></JournalIssue><Title>Genetics and molecular research : GMR</Title><ISOAbbreviation>Genet Mol Res</ISOAbbreviation></Journal><ArticleTitle>Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.4238/gmr.15028635</ELocationID><Abstract><AbstractText>We characterized new transgenic varieties of poplar with multiple insect-resistant and salt stress tolerant genes. Two insect-resistant Bacillus thuringiensis (Bt) genes, Cry1Ac and Cry3A, and a salt-tolerant gene, Betaine aldehyde dehydrogenase (BADH) were inserted into a vector, p209-Cry1Ac-Cry3A-BADH. The clone of Populus x euramericana was transformed by the vector using the Agrobacterium-mediated method. Three transgenic lines were assessed using genetic detection and resistance expression analysis. PCR revealed that exogenous genes Cry1Ac, Cry3A, BADH and selective marker gene NPTII were present in three transgenic lines. Quantitative real-time PCR (qPCR) showed significant differences in the transcriptional abundance of three exogenous genes in different lines. Results of assays for Bt toxic proteins showed that the Cry1Ac and Cry3A toxic protein content of each line was 12.83-26.32 and 2108.91-2724.79 ng/g, respectively. The Cry1Ac toxic protein content of different lines was significantly different; the Cry3A toxic protein content was about 100 times higher than that of the Cry1Ac toxic protein. The insect-resistance test revealed the mortality rate of transgenic lines to Hyphantria cunea L1 larvae varied by 42.2-66.7%, which was significantly higher than non-transgenic lines. The mortality rate of L1 and L2 Plagiodera versicolora larvae was 100%. The insecticidal effect of transgenic lines to P. versicolora larvae was higher than that to H. cunea larvae. NaCl stress tolerance of three transgenic lines under 3-6% NaCl concentration was significantly higher than that of non-transgenic lines. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>R L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>A X</ForeName><Initials>AX</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>M S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>J M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Genet Mol Res</MedlineTA><NlmUniqueID>101169387</NlmUniqueID><ISSNLinking>1676-5680</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001427">Bacterial Toxins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001413" MajorTopicYN="N">Bacillus thuringiensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001427" MajorTopicYN="N">Bacterial Toxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055049" MajorTopicYN="N">Salt Tolerance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="Y">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27173305</ArticleId><ArticleId IdType="pii">gmr8635</ArticleId><ArticleId IdType="doi">10.4238/gmr.15028635</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yang, R L" sort="Yang, R L" uniqKey="Yang R" first="R L" last="Yang">R L Yang</name></noRegion><name sortKey="Dong, Y" sort="Dong, Y" uniqKey="Dong Y" first="Y" last="Dong">Y. Dong</name><name sortKey="Dong, Y" sort="Dong, Y" uniqKey="Dong Y" first="Y" last="Dong">Y. Dong</name><name sortKey="Wang, A X" sort="Wang, A X" uniqKey="Wang A" first="A X" last="Wang">A X Wang</name><name sortKey="Wang, A X" sort="Wang, A X" uniqKey="Wang A" first="A X" last="Wang">A X Wang</name><name sortKey="Wang, J M" sort="Wang, J M" uniqKey="Wang J" first="J M" last="Wang">J M Wang</name><name sortKey="Wang, J M" sort="Wang, J M" uniqKey="Wang J" first="J M" last="Wang">J M Wang</name><name sortKey="Yang, M S" sort="Yang, M S" uniqKey="Yang M" first="M S" last="Yang">M S Yang</name><name sortKey="Yang, M S" sort="Yang, M S" uniqKey="Yang M" first="M S" last="Yang">M S Yang</name><name sortKey="Yang, R L" sort="Yang, R L" uniqKey="Yang R" first="R L" last="Yang">R L Yang</name><name sortKey="Zhang, J" sort="Zhang, J" uniqKey="Zhang J" first="J" last="Zhang">J. Zhang</name><name sortKey="Zhang, J" sort="Zhang, J" uniqKey="Zhang J" first="J" last="Zhang">J. 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|texte= Genetic transformation and expression of transgenic lines of Populus x euramericana with insect-resistance and salt-tolerance genes.
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Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27173305" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |